nos [2019-06-25 10:32:44]

nitric-oxide synthase

Locus: BSU07630

Isoelectric point: 5.53

Molecular weight: 38.59 kDa

Protein length: 363 aa

Gene length: 1092 bp

Function: production of nitric oxide

Product: nitric-oxide synthase

Essential: no

Synonyms: yflM

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds

Gene

Coordinates: 836,653 → 837,744

The protein

Catalyzed reaction/ biological activity

• NADPH-dependent oxidation of l-arginine into l-citrulline PubMed

Protein family

• Bacterial NOS oxygenase subfamily (according to Swiss-Prot)

Cofactors

• heme b PubMed
• NADPH PubMed
• FMN or FAD PubMed

Structure

• 2AMO (loose dimer), 1M7V (bound to tetrahydrofolate and arginine)

Expression and Regulation

Operons

• Genes: nos-yflK
  Description: PubMed

  Additional information

  • Note that the yflL is located between nos and yflK, but on the opposite strand

Biological materials

Mutant

• MGNA-C254 (yflM::erm), available at the NBRP B. subtilis, Japan
• BKE07630 (Δnos::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAAACTCTCCCCTTCAC, downstream forward: _UP4_AACTATTTTTATCAAGATAA
• BKK07630 (Δnos::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACAAACTCTCCCCTTCAC, downstream forward: _UP4_AACTATTTTTATCAAGATAA

References

Reviews

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Original publications

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